Dr. Nour Moussa article in JAS: Reports

Abstract: The Lake Baikal region of Siberia was home to two temporally distinct populations from Early Neolithic, EN (7500–7000 cal BP) to Late Neolithic-Early Bronze Age, LN-EBA (5570–3725 cal BP). The EN group was separated from the LN-EBA group by a ~ 1500-year gap (hiatus), and during this hiatus no human remains have been recovered from the Lake Baikal area. Examination of the paternal lineage through Y-chromosomal polymorphisms is a novel approach to BAP and will facilitate the assessment of the paternal continuities and/or discontinuities within and between the EN and the LN-EBA groups, and complement the previously examined maternal data. Several new ancient DNA extraction and PCR amplification techniques were optimized to address the technical challenges during sample analysis. Each sample was extracted twice in duplicate on different occasions to authenticate the results. Thirteen Y-chromosomal Single Nucleotide Polymorphism (SNP) markers were examined via the SNaPshot multiplex PCR reaction to determine Y-chromosomal haplogroups of males. Results have been obtained from 16 males from the EN cemeteries Lokomotiv and Shamanka II representing haplogroups K, R1a1 and C3, and 20 males from the LN-EBA Ust’-Ida and Kurma XI cemeteries representing haplogroups Q, K and unidentified SNP (L914). For those males belonging to haplogroup Q, further experiments were obtained to examine sub-haplogroups of Q, and the results showed that those males belong to sub-haplogroup Q1a3. The paternal Y-chromosome results suggest a discontinuity between the EN and LN-EBA populations. The significance of this research lies on the utility of DNA analysis in making inferences about the pre-historic social structure.